Team:Tokyo Tech
From 2011.igem.org
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<h2 id="Overall"> Overview: Cool down in summer with our Rock-Paper-Scissors Game</h2> | <h2 id="Overall"> Overview: Cool down in summer with our Rock-Paper-Scissors Game</h2> | ||
- | <img src="https://static.igem.org/mediawiki/2011/2/24/Happy-rps.png" width=" | + | <img src="https://static.igem.org/mediawiki/2011/2/24/Happy-rps.png" width="408px" alt="Project Overview" style="float:right;" /> |
<p> | <p> | ||
- | When summer comes it brings vacations, so students have plenty time to have fun. | + | When summer comes it brings vacations, so students have plenty time to have fun. |
+ | But summer means hot weather! In light of these circumstances, | ||
+ | we designed a game that can be played between<span class="name"> E.coli</span> and humans, | ||
+ | and that the winner of the game can get refreshing prizes! | ||
+ | Dear students, let us introduce you to the first human-bacteria Rock-Paper-Scissors game! | ||
+ | Win and get a rain shower, plus a “urea cooler”! | ||
</p> | </p> | ||
- | <h2 id="RPS"> Rock-Paper-Scissors (RPS) Game</h2> | + | <h2 id="RPS"> Rock-Paper-Scissors (RPS) Game </h2> |
- | <img src="https://static.igem.org/mediawiki/2011/1/10/TokyoTech_home_fig2.png" alt="RPS" class="fig" /> | + | <img src="https://static.igem.org/mediawiki/2011/1/10/TokyoTech_home_fig2.png" alt="RPS" width="480px" class="fig" /> |
<p> | <p> | ||
- | So, how to play RPS with a handless bacteria? Instead of hands, we use different signaling molecules corresponding either to rock, paper or scissors. In our set of six signaling molecules, humans use IPTG, aTc and salicylate, and E. coli uses 3O-C6-HSL, 3O-C12-HSL and AI-2. In each case, these signaling molecules correspond to rock, paper and scissors respectively. | + | So, how to play RPS with a handless bacteria? |
+ | Instead of hands, we use different signaling molecules corresponding | ||
+ | either to rock, paper or scissors. | ||
+ | In our set of six signaling molecules, humans use IPTG, | ||
+ | aTc and salicylate, and <span class="name">E. coli</span> uses | ||
+ | 3O-C6-HSL, 3O-C12-HSL and AI-2. In each case, | ||
+ | these signaling molecules correspond to rock, paper and scissors respectively. | ||
</p> | </p> | ||
+ | |||
<p> | <p> | ||
- | The next step is to design a way to know who wins the RPS game. That is when the< | + | The next step is to design a way to know who wins the RPS game. |
+ | That is when the<span class="name"> E. coli</span> judge team comes in! | ||
+ | Each judge has an AND-gate promoter which produces a single output | ||
+ | based on two inputs, human side and<span class="name"> E.coli</span> | ||
+ | side signaling molecules. Since we want the output to be visible, | ||
+ | we use either GFP, RFP or CFP to indicate whether humans win, | ||
+ | lose or it is a tie, respectively. | ||
</p> | </p> | ||
+ | |||
<p> | <p> | ||
- | + | Lastly, we need to make sure <span class="name">E. coli</span> follows | |
+ | the rules of the game by synthetizing only one signaling molecule | ||
+ | every time it plays. Importantly, we have to design the opponent | ||
+ | <span class="name">E. coli</span> to be able to choose its signal | ||
+ | randomly for a fare game, because <span class="name">E. coli</span> | ||
+ | will continue to lose if <span class="name">E. coli</name> always | ||
+ | produce same signaling molecule. In a view of these needs, | ||
+ | we designed three randomizers that satisfy the conditions for the game. | ||
+ | Single Colony Isolation, Survival of single strain and Conditional Knockout. | ||
+ | <a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/index.htm">(see more...)</a> | ||
</p> | </p> | ||
- | <h2 id="rain"> Make it Rain</h2> | + | <h2 id="rain"> Make it Rain </h2> |
- | <img src="https://static.igem.org/mediawiki/2011/7/7b/TokyoTech_home_fig3.png" alt="Raining" class="fig" /> | + | <img src="https://static.igem.org/mediawiki/2011/7/7b/TokyoTech_home_fig3.png" alt="Raining" width="480px" class="fig" /> |
<p> | <p> | ||
- | Playing RPS with < | + | Playing RPS with <span class="name">E. coli</span> during summer was fun, but, |
+ | even if humans won, pleasures did not last long since we soon returned to | ||
+ | complaining about the hot weather. As a prize for humans who win in our RPS game, | ||
+ | we designed an <span class="name">E. coli</span> that can make it rain, | ||
+ | making the hot summer more fun and refreshing (let alone applications in agriculture). | ||
+ | To make it rain, we constructed an isoprene synthetizing <span class="name">E. coli</span>. | ||
+ | Photo-oxidized isoprene acts as a condensation nucleus(Colin D. O‘Dowd <i> et al</i>., 2002, Nature), | ||
+ | which might cause rain, even though it is present in very low concentrations. | ||
+ | <a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm">(see more...)</a> | ||
</p> | </p> | ||
- | <h2 id="urea">Urea Coolers</h2> | + | <h2 id="urea"> Urea Coolers </h2> |
- | <img src="https://static.igem.org/mediawiki/2011/e/ef/Home-urea.png" alt="Urea cooler" class="fig" /> | + | <img src="https://static.igem.org/mediawiki/2011/e/ef/Home-urea.png" alt="Urea cooler" width="480px" class="fig" /> |
<p> | <p> | ||
- | + | Coolers can be made by adding urea to water, since dissolving urea in water | |
- | + | is an endothermic reaction (-57.8 cal/g). We can make <span class="name">E.coli</span> | |
- | + | synthetize urea just by introducing a gene encoding arginase! | |
- | + | </p> | |
+ | <p> | ||
+ | To analyze how we obtain even more urea from our <span class="name">E. coli</span>, | ||
+ | elementary flux analysis (Schuste<i>et al.</i> 2000) identified metabolic routes | ||
+ | that are both stoichiometrically and thermodynamically feasible for a group of enzymes. | ||
+ | Making use of it we can tell which substrates and pathways are the best for obtaining | ||
+ | more urea.<a href="https://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm">(see more...)</a> | ||
</p> | </p> | ||
- | <h2 id="manabi"> Human Practice</h2> | + | <h2 id="manabi"> Human Practice </h2> |
- | + | <img src="https://static.igem.org/mediawiki/2011/2/22/Manabi-ppt2.png" alt="Creating Perception" width="480px" class="fig" /> | |
- | + | <p> | |
- | + | We love Synthetic Biology, and to share our passion with other people, | |
- | + | we did educational activities as part of our human practices. | |
- | + | We would like to spread what is made and aimed by synthetic biology and iGEM. | |
- | + | For this event, we created “iGEM Card Game”, posters and questionnaire. | |
+ | <a href="https://2011.igem.org/Team:Tokyo_Tech/HumanPractice.htm">(see more...)</a> | ||
+ | </p> | ||
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Revision as of 07:26, 5 October 2011
iGEM Tokyo Tech 2011 Team
Overview: Cool down in summer with our Rock-Paper-Scissors Game
When summer comes it brings vacations, so students have plenty time to have fun. But summer means hot weather! In light of these circumstances, we designed a game that can be played between E.coli and humans, and that the winner of the game can get refreshing prizes! Dear students, let us introduce you to the first human-bacteria Rock-Paper-Scissors game! Win and get a rain shower, plus a “urea cooler”!
Rock-Paper-Scissors (RPS) Game
So, how to play RPS with a handless bacteria? Instead of hands, we use different signaling molecules corresponding either to rock, paper or scissors. In our set of six signaling molecules, humans use IPTG, aTc and salicylate, and E. coli uses 3O-C6-HSL, 3O-C12-HSL and AI-2. In each case, these signaling molecules correspond to rock, paper and scissors respectively.
The next step is to design a way to know who wins the RPS game. That is when the E. coli judge team comes in! Each judge has an AND-gate promoter which produces a single output based on two inputs, human side and E.coli side signaling molecules. Since we want the output to be visible, we use either GFP, RFP or CFP to indicate whether humans win, lose or it is a tie, respectively.
Lastly, we need to make sure E. coli follows the rules of the game by synthetizing only one signaling molecule every time it plays. Importantly, we have to design the opponent E. coli to be able to choose its signal randomly for a fare game, because E. coli will continue to lose if E. coli always produce same signaling molecule. In a view of these needs, we designed three randomizers that satisfy the conditions for the game. Single Colony Isolation, Survival of single strain and Conditional Knockout. (see more...)
Make it Rain
Playing RPS with E. coli during summer was fun, but, even if humans won, pleasures did not last long since we soon returned to complaining about the hot weather. As a prize for humans who win in our RPS game, we designed an E. coli that can make it rain, making the hot summer more fun and refreshing (let alone applications in agriculture). To make it rain, we constructed an isoprene synthetizing E. coli. Photo-oxidized isoprene acts as a condensation nucleus(Colin D. O‘Dowd et al., 2002, Nature), which might cause rain, even though it is present in very low concentrations. (see more...)
Urea Coolers
Coolers can be made by adding urea to water, since dissolving urea in water is an endothermic reaction (-57.8 cal/g). We can make E.coli synthetize urea just by introducing a gene encoding arginase!
To analyze how we obtain even more urea from our E. coli, elementary flux analysis (Schusteet al. 2000) identified metabolic routes that are both stoichiometrically and thermodynamically feasible for a group of enzymes. Making use of it we can tell which substrates and pathways are the best for obtaining more urea.(see more...)
Human Practice
We love Synthetic Biology, and to share our passion with other people, we did educational activities as part of our human practices. We would like to spread what is made and aimed by synthetic biology and iGEM. For this event, we created “iGEM Card Game”, posters and questionnaire. (see more...)