Team:Tec-Monterrey/projectresults

From 2011.igem.org

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         <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectoverview">overview</a></p>
         <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectoverview">overview</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectparts">parts</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectparts">parts</a></p>
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             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">modeling</a></p>
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             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/projectmodeling">genetic frame</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/safetypage">safety</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/safetypage">safety</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human practice</a></p>
             <p><a href="https://2011.igem.org/Team:Tec-Monterrey/teamha">human practice</a></p>

Revision as of 12:12, 28 September 2011

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There are several tecniques to prove the successful transportation of CelD and SacC into the outer membrane of E. coli. In this project, SDS-PAGE of entire cell culture samples, SDS-PAGE of membrane fraction samples, and measurement of enzyme activity of whole-cell-system without any chemical purification operation have been considered in order to confirm the presence of active enzymes on the external membrane of E. coli.