Team:Bilkent UNAM Turkey/Experiment
From 2011.igem.org
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We sent genes to sequencing and sequencing data should be available on their <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Bilkent_UNAM_Turkey">link</a>. | We sent genes to sequencing and sequencing data should be available on their <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=Bilkent_UNAM_Turkey">link</a>. | ||
- | + | Polymerase Chain Reaction of GFP from pKScGFP | |
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+ | For 1.0µl of DNA we added, | ||
+ | • 1.0µl of forward primer | ||
+ | • 1.0µl of reverse primer | ||
+ | • 2.5µl of 10X PCR Buffer | ||
+ | • 0.5µl of MgSO4 | ||
+ | • 0.5µl of dNTP mix | ||
+ | • 18.375µl of ddH2O | ||
+ | • 0.125µl of Taq polymerase | ||
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+ | Parameters in thermal cycler were chosen as below: | ||
+ | Denaturation is 95oC for 4min | ||
+ | Annealing is 30 cycles: | ||
+ | • 95oC 1min | ||
+ | • 65oC 1min | ||
+ | • 72oC 2min | ||
+ | Extension 72oC for 10min | ||
+ | 4oC hold | ||
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+ | GFP fusion | ||
+ | GFP fusion with nfsI gene on our Biobrick is our plannned future work. | ||
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Revision as of 21:51, 21 September 2011
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