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== Notebook: Week 3 == | == Notebook: Week 3 == | ||
Monday - Food
Tuesday - Darren arrives
- Discussion of R. inquisitor
Wednesday - Tokyo Tech iGEM Plasmid (TmAFP) and Canada, Davies Lab Plasmid (ZeAFP) arrived, streaked 1 plate for each plasmid
- Modelling/homology study for RiAFP
- Afternoon meeting with Prof. Farren Isaacs about project and gene we're looking to synthesize
- Emailed companies for sponsorship for gene synthesis
- Emailed other iGEM teams for suggestion
Thursday - Found protocol for Origami cell lines at Xiong lab
- Designed gene on IDT website
Friday - Asking about TEV, GFP, His
- Ordered gene
- Contacted Israeli lab about Plasmids
Saturday - Miniprep, Nanodrop
- Cultured 2 colonies in 100 mL flasks
- Warren worked on primer design
Sunday - 1/40 dilution in 1 liter
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