Team:Peking S

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A ‘Chemical Wire’ Toolbox for Synthetic Microbial Consortia

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Click below To access a brief introduction of our project

Project presentation
Cell-cell communication-based multicellular networks provide an extended vista for synthetic biology. However, ‘chemical wires’ that allow versatile concurrent communications are far from sufficient. Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression systems have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density has been created with supporting microfluid device.
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-'''PROJECT SUMMARY'''
+<div id="backtoigem2011" style="position:absolute; overflow:hidden; left:770px; top:300px; width:200px; height:200px; z-index:23">
+<font face="verdana "class="ws14" color="#ffffff">TO IGEM HQ!
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-Cell-cell communication based multi-cellular systems provide an extended vista for synthetic biology. By compartmentalizing the target network into different cells, crosstalk between molecular interactions and metabolic burden of single cells may be reduced and increasingly complex dynamic functions might be more realizable. However, well-characterized orthogonal signaling systems are far from sufficient.
-Accordingly, our project intends to develop a versatile signaling platform for complicated multicellular dynamics. For this goal, we are going to characterize a set of recently reported quorum sensing molecules to implement cell-cell communication in a variety of forms. Further, we are constructing logic gates and a teamwork based cell density oscillator to test our Biobricks in both Boolean andntinuous networks.
+<div id="text1" style="position:absolute; overflow:hidden; left:130px; top:365px; width:700px;z-index:25;">
+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>A </span><span style="font-size:16px"><font color="#ffffff" class="ws16">&#8216;Chemical Wire&#8217; Toolbox for Synthetic Microbial Consortia</B></font></span></div>
+<div id="text3" style="position:absolute; overflow:hidden; left:160px; top:1070px; z-index:27;">
+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Q</span><span style="font-size:16px"><font color="#ffffff" class="ws16">uick Links</B></font></span></div>
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-   <a href="https://2011.igem.org/Team:Peking_S/lab/biosafety" class="project"><span class="project">GO TO SAFETY PAGE</span></a></p><p class="mainbody">&nbsp;</p></th>
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+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>S</span><span style="font-size:16px"><font color="#ffffff" class="ws16">ponsed By</B></font></span></div>
+<div id="text5" style="position:absolute; overflow:hidden; left:120px; top:750px;width:200px z-index:100;">
+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Click below</span><span style="font-size:16px"><font color="#000000" class="ws16"> To access a brief introduction of our project<br><br></B></font></span></div>
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+<b>Project presentation</b>
+<br>
+</a>
+<b>Cell-cell communication</b>-based multicellular networks provide an extended vista for synthetic biology. However, ‘chemical wires’ that allow versatile concurrent communications are far from sufficient.
+Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression systems have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density has been created with supporting microfluid device.
+<br>
+<a href=https://2011.igem.org/Team:Peking_S/project>
+learn more
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+<div id="image1" style="position:absolute; overflow:hidden; left:489px; top:1134px; width:292px; height:37px; z-index:40"><a href="http://www.phy.pku.edu.cn/English.html"><img src="https://static.igem.org/mediawiki/2011/7/7e/Pkuphy1.png"  alt="" title="" border=0 width=292 height=37></div>
+<div id="image2" style="position:absolute; overflow:hidden; left:501px; top:1230px; width:294px; height:36px; z-index:41"><a href="http://ctb.pku.edu.cn/main/en/index.htm"><img src="https://static.igem.org/mediawiki/2011/8/8e/PekingR_PKU_CTB.gif" alt="" title="" border=0 width=294 height=36></div>
+<div id="image3" style="position:absolute; overflow:hidden; left:697px; top:1182px; width:194px; height:43px; z-index:42"><a href="http://dean.pku.edu.cn/"><img src="https://static.igem.org/mediawiki/2011/2/20/PekingR_PKU_OFFICE_OF_EDUCATIONAL_ADMINISTRATION_%281%29.png" alt="" title="" border=0 width=194 height=43></div>
+<div id="image4" style="position:absolute; overflow:hidden; left:661px; top:1276px; width:255px; height:43px; z-index:43"><a href="http://www.bio.pku.edu.cn/english/"><img src="https://static.igem.org/mediawiki/2011/7/72/PekingR_PKU_SCHOOL_OF_LIFE_SCIENCE.png" alt="" title="" border=0 width=255 height=43></div>
+<div id="image5" style="position:absolute; overflow:hidden; left:423px; top:1275px; width:232px; height:44px; z-index:44"><a href="http://www.bio.pku.edu.cn/syzx/">
+<img src="https://static.igem.org/mediawiki/2011/4/4b/PekingR_PKU_OFFICE_OF_EDUCATIONAL_ADMINISTRATION_%282%29.png"  alt="" title="" border=0 width=232 height=44></div>
+<div id="image7" style="position:absolute; overflow:hidden; left:455px; top:1182px; width:230px; height:37px; z-index:45"><a href="http://www.beijinglab.com.cn/"><img src="https://static.igem.org/mediawiki/2011/e/ec/PekingR_beijinglihua.jpg" alt="" title="" border=0 width=230 height=37></div>
+<div id="image8" style="position:absolute; overflow:hidden; left:472px; top:1325px; width:250px; height:35px; z-index:46"><a href="http://web5.pku.edu.cn/huanjing/"><img src="https://static.igem.org/mediawiki/2011/0/02/PekingR_COLLEGE_OF_ENVIRONMENTAL_SCIENCES_AND_ENGINEERING_%281%29.png" alt="" title="" border=0 width=250 height=35></div>
+<div id="image9" style="position:absolute; overflow:hidden; left:526px; top:1366px; width:245px; height:45px; z-index:47"><a href="http://www.biolog.de/home/"><img src="https://static.igem.org/mediawiki/2011/c/c8/PekingR_BIOLOG.gif"alt="" title="" border=0 width=245 height=45></div>
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