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Team:KULeuven/Protocols
From 2011.igem.org
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<h2>Transformation of <i>E. Coli</i></h2> | <h2>Transformation of <i>E. Coli</i></h2> | ||
| - | + | <b>Preparing a cell structure</b><br> | |
| + | At present we use TOP10F<sup>1</sup> cells which allow both plasmid and M13 bacteriophage transformation (For M13 transformation you need the F<sup>1</sup> plasmid for absorption of the bacteriophages).<br><br> | ||
</div> | </div> | ||
Revision as of 10:50, 19 August 2011
Protocols
Transformation of E. Coli
Preparing a cell structureAt present we use TOP10F1 cells which allow both plasmid and M13 bacteriophage transformation (For M13 transformation you need the F1 plasmid for absorption of the bacteriophages).
