Team:Paris Liliane Bettencourt/Notebook/2011/08/18/

From 2011.igem.org

(Difference between revisions)
(→Restarting since the PCR Purification)
(→Restarting since the PCR Purification)
Line 7: Line 7:
*Digestion with ES and XS only for LacI/GFP
*Digestion with ES and XS only for LacI/GFP
[[File:geldigky2.jpg|450px|thumb|center|Gel of digestion - Column 1.2 - 1.3 - 2.2 - 2.3 - 3.7 - 3.8 - 4.7 - 4.8 / S27 digested ES]]
[[File:geldigky2.jpg|450px|thumb|center|Gel of digestion - Column 1.2 - 1.3 - 2.2 - 2.3 - 3.7 - 3.8 - 4.7 - 4.8 / S27 digested ES]]
-
*TECAN  
+
*Gel extraction and TECAN  
*Ligation
*Ligation

Revision as of 18:19, 18 August 2011

Kevin

Results from sequencing

There a mistake, it seems that we have the T amber instead of Lac/Tet stuff ... Let's restart again to make sure of it.

Restarting since the PCR Purification

  • Digestion with ES and XS only for LacI/GFP
Gel of digestion - Column 1.2 - 1.3 - 2.2 - 2.3 - 3.7 - 3.8 - 4.7 - 4.8 / S27 digested ES
  • Gel extraction and TECAN
  • Ligation
Retrieved from "http://2011.igem.org/Team:Paris_Liliane_Bettencourt/Notebook/2011/08/18/"