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Team:Freiburg/Notebook/14 July
From 2011.igem.org
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Revision as of 14:54, 18 August 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
Contents |
red light receptor
3A-assembly: Digest
Investigators: Jakob
3A-assembly of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator)
S21a:
- Enzyme 1: XbaI
- Enzyme 2: PstI
S22, S23
- Enzyme 1: EcoRI
- Enzyme 2: SpeI
Vector:
- Enzyme 1: EcoRI
- Enzyme 2: PstI
- Enzyme 3: DpnI
3A-assembly: Ligation and transformation
Investigators: Julia
Ligation of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator) in vector pSB1T3.
L23: Ligation of S23+S21a in pSB1T3 L22: Ligation of S22+S21a in pSB1T3
Plasmids were transformed in cells and plated out.
PCR
Investigators: Julia
PCR of the red light receptor, cph8.
Primer:
- P 10
- P 11
DNA template:
- BBa_I150010
Lysis cassette
Precipitator
Test-digest
Investigators: Rüdiger
Testdigest of the minipreps, Sophie did (june, 13).
- Enzyme 1: PstI
- Enzyme 2: EcoRI
Image of different samples from miniprep.
