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Revision as of 02:34, 14 August 2011 (view source) Alxcia (Talk | contribs) (→Notebook) ← Older edit		Revision as of 06:25, 14 August 2011 (view source) Arj002 (Talk | contribs) (→Notebook) Newer edit →
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	==Notebook==		==Notebook==
-	You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.	+
	-LB media Method-		-LB media Method-
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Revision as of 06:25, 14 August 2011

Notebook

-LB media Method- Dissolve 10g trytone, 5g yeast extract and 10g NaCl in 800 mL MilliQ water, making use of a magnetic stirrer. Once dissolved, bring volume up to 1 litre using the MilliQ water. Autoclave 500 mL of the solution (121oC, 15 min, standard liquid cycle).

-LB agar Method- Add 7.5 g Bacto agar to the remaining 500 mL of LB media and autoclave [121oC, 15 min, standard liquid cycle]. Add 250 µL of Chloroamphenicol and mix well before plating out and setting agar.