Team:Harvard/Template:NotebookData

From 2011.igem.org

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__NOTOC__
__NOTOC__
==June 6th==
==June 6th==
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First day of iGEM.</div>
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First day of iGEM!</div>
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<div id="607" style="display:none">
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== June 7th ==
== June 7th ==
'''Miniprep of pKD42 (lambda red)'''
'''Miniprep of pKD42 (lambda red)'''
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*7) 4⁰C forever</div>
*7) 4⁰C forever</div>
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<div id="609" style="display:none">
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== June 9th ==
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== June 9th - Wet Lab ==
*Created cell culture with selection construct (contains ZFB, His3, pyrF on plasmid) and reporter RFP (this will be used to test positive control ZFs, cells fluoresce green when ZF binds)
*Created cell culture with selection construct (contains ZFB, His3, pyrF on plasmid) and reporter RFP (this will be used to test positive control ZFs, cells fluoresce green when ZF binds)
**Picked colonies, grew in LB/amp liquid media until mid-log
**Picked colonies, grew in LB/amp liquid media until mid-log
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*Plated selection strain from gel stab onto tet plate.
*Plated selection strain from gel stab onto tet plate.
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*Began primer design for creating the kan/selection construct fusion (see our [[Primers | primer spreadsheet]] for details).
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*Began primer design for creating the kan/selection construct fusion.
==June 9th - Bioinformatics==
==June 9th - Bioinformatics==
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Follow up work on this will be to convert this table to frequencies instead of values: values are less meaningful. </div>
Follow up work on this will be to convert this table to frequencies instead of values: values are less meaningful. </div>
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<div id="610" style="display:none">
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== June 10th ==
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== June 10th - Wet Lab ==
*What we learned today: don't put E. coli plates in the -20C freezer!
*What we learned today: don't put E. coli plates in the -20C freezer!
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Follow up work here is to check more properties, and maybe try individual pairings (ex. phobic-philic, polar-phillic).</div>
Follow up work here is to check more properties, and maybe try individual pairings (ex. phobic-philic, polar-phillic).</div>
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<div id="613" style="display:none">
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== June 13th ==
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== June 13th - Wet Lab ==
The control zinc fingers OZ052 and OZ123 were amplified with overhanging primers to allow its insertion into the Wolfe plasmid:
The control zinc fingers OZ052 and OZ123 were amplified with overhanging primers to allow its insertion into the Wolfe plasmid:

Latest revision as of 14:52, 12 August 2011