"
Team:British Columbia/Notebook/Week 8
From 2011.igem.org
| Line 14: | Line 14: | ||
[[File:Marijacob3.JPG]] | [[File:Marijacob3.JPG]] | ||
| + | |||
| + | Alpha- Pinene | ||
| + | |||
| + | July 30th | ||
| + | |||
| + | Joe finally received his primers for PCR amplification of his SDM-alpha pinene synthase necessary for the ligation into yeast and biobrick plasmids. This weekend, he PCR his | ||
Revision as of 05:21, 2 August 2011
On July 25th, Gurpal performed a yeast transformation. He transformed his purified pBS and pKS into 2 separate wildtype yeasts. He followed a protocol, left the cells to incubate at 30 degrees Celsius at 3:10pm, heat shocked them for 20 minutes at 42 degrees Celsius and then plated them at 5:30pm. The plates were marked with URA so that the plasmids could grow on them. Then, they were incubated in a 30 degree Celsius incubator.
On July 27th, Alina observed the cells and noted that the transformation was a success.
July 26 2011
Marianne and Jacob are putting their parts in the biobricks plasmids!
Alpha- Pinene
July 30th
Joe finally received his primers for PCR amplification of his SDM-alpha pinene synthase necessary for the ligation into yeast and biobrick plasmids. This weekend, he PCR his
