Team:Freiburg/Notebook/14 July
From 2011.igem.org
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'''Investigators: Jakob''' | '''Investigators: Jakob''' | ||
- | 3A-assembly of S22+S21a and S23+S21a | + | 3A-assembly of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator) |
+ | |||
+ | S21a: | ||
+ | *Enzyme 1: XbaI | ||
+ | *Enzyme 2: PstI | ||
+ | |||
+ | S22, S23 | ||
+ | *Enzyme 1: EcoRI | ||
+ | *Enzyme 2: SpeI | ||
+ | |||
+ | Vector: | ||
+ | *Enzyme 1: EcoRI | ||
+ | *Enzyme 2: PstI | ||
+ | *Enzyme 3: DpnI | ||
+ | |||
+ | ===3A-assembly: Ligation and transformation=== | ||
+ | |||
+ | '''Investigators: Julia''' | ||
+ | |||
+ | Ligation of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator) in vector pSB1T3. | ||
+ | |||
+ | L23: Ligation of S23+S21a in pSB1T3 | ||
+ | L22: Ligation of S22+S21a in pSB1T3 | ||
+ | |||
+ | Plasmids were transformed in cells and plated out. | ||
==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== |
Revision as of 13:49, 15 July 2011
Contents |
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
3A-assembly: Digest
Investigators: Jakob
3A-assembly of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator)
S21a:
- Enzyme 1: XbaI
- Enzyme 2: PstI
S22, S23
- Enzyme 1: EcoRI
- Enzyme 2: SpeI
Vector:
- Enzyme 1: EcoRI
- Enzyme 2: PstI
- Enzyme 3: DpnI
3A-assembly: Ligation and transformation
Investigators: Julia
Ligation of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator) in vector pSB1T3.
L23: Ligation of S23+S21a in pSB1T3 L22: Ligation of S22+S21a in pSB1T3
Plasmids were transformed in cells and plated out.
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
Test-digest
Investigators: Rüdiger
Testdigest of the minipreps, Sophie did (june, 13).
- Enzyme 1: PstI
- ENzyme 2: EcoRI
Image of different samples from miniprep.