"
Team:Freiburg/Notebook/18 June
From 2011.igem.org
(Difference between revisions)
(→3A assembly: Ligation) |
(→Transformation) |
||
| Line 12: | Line 12: | ||
Target vector | Target vector | ||
| - | ==Transformation== | + | ===Transformation=== |
'''Investigators: Theo''' | '''Investigators: Theo''' | ||
Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor. | Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor. | ||
Revision as of 09:45, 15 July 2011
Lysis cassette
3A assembly: Ligation
Investigators: Theo
Comments: Ligation of digested parts from yesterday.
S11: heat sensitive cI QPI with a high promoter preceding the repressor
S15: Bacteriophage Holin Gene pS105
S1, S2, S3: promotor
S4, S5, S6: RBS
Target vector
Transformation
Investigators: Theo
Transformation of ligated parts. Incubation at 30°C because of the heat sensitive promotor.
