Team:Tsinghua-A/Parts

From 2011.igem.org

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{{:Team:Tsinghua-A/template/killbanner}}
{{:Team:Tsinghua-A/template/killbanner}}
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<html xmlns="http://www.w3.org/1999/xhtml">
<head>
<head>
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<!-----ywgiyrryyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyy---->
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<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1" />
 +
<title>JavaScript Slideshow - TinySlideshow</title>
 +
<link rel="stylesheet" href="http://bioinfo.au.tsinghua.edu.cn/member/IGEM/thu_style.css" />
<style>
<style>
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body {
body {
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 +
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font-family:"Times New Roman",Georgia,Serif;
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/* Menu */
/* Menu */
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padding-left: 0;
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</style>
 
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<!-----ywgiyrryyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyyy---->
 
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<meta name="keywords" content="" />
 
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<meta name="description" content="" />
 
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<meta http-equiv="content-type" content="text/html; charset=utf-8" />
 
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<title>Republic  by Free CSS Templates</title>
 
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<!--link href="style.css" rel="stylesheet" type="text/css" media="screen" />
 
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<!--11111111111111111111111111111111111111111111111111111111111111-->
 
</head>
</head>
<body>
<body>
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<div id="wrapper">
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<div style="position:fixed;right:0px;bottom: 0px;display:inline"><a href="#back-top">
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  <div id="header">
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<img src="https://static.igem.org/mediawiki/2011/5/52/Back-top.png" width="60px" height="60px"></a></div>
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<div id="logo">
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<p id="back-top" align="CENTER" STYLE="text-indent:0em"><img src="https://static.igem.org/mediawiki/2011/1/17/THU_banner_par.jpg" alt="" width="960"/></p>
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<p align="left"><img src="https://static.igem.org/mediawiki/2011/5/5e/Thubanner.jpg" alt="" width="960"/></p>
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</div>
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</div>
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<!-- end #header -->
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<div id="menu">
<div id="menu">
<ul>
<ul>
-
<li ><a href="https://2011.igem.org/Team:Tsinghua-A/Team">Team</a></li>
+
<li><a href="https://2011.igem.org/Team:Tsinghua-A/Team">Team</a></li>
-
<li ><a href="https://2011.igem.org/Team:Tsinghua-A/Project">Project</a></li>
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<li><a href="https://2011.igem.org/Team:Tsinghua-A/Project">Project</a></li>
<li><a href="https://2011.igem.org/Team:Tsinghua-A/Safety">Safety</a></li>
<li><a href="https://2011.igem.org/Team:Tsinghua-A/Safety">Safety</a></li>
-
<li><a href="https://2011.igem.org/Team:Tsinghua-A/Modeling">Modeling</a></li>
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<li ><a href="https://2011.igem.org/Team:Tsinghua-A/Modeling">Modeling</a></li>
<li class="current_page_item"><a href="https://2011.igem.org/Team:Tsinghua-A/Parts">Parts</a></li>
<li class="current_page_item"><a href="https://2011.igem.org/Team:Tsinghua-A/Parts">Parts</a></li>
<li ><a href="https://2011.igem.org/Team:Tsinghua-A/Notebook">Notebook</a></li>
<li ><a href="https://2011.igem.org/Team:Tsinghua-A/Notebook">Notebook</a></li>
</ul>
</ul>
</div>
</div>
-
<p>&nbsp;</p>
 
-
<!-- end #menu -->
 
-
<div id="page">
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<!--end of enu--------------------------------------------><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
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<div id="page-bgtop">
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<div id="page-bgbtm"><span class="title"><a href="https://2011.igem.org/Team:Tsinghua-A"><span class="meta">
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-
<img src="https://static.igem.org/mediawiki/2011/c/c6/Thua_bann.jpg" alt="" width="960" height="328" /></span></a></span>
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<div id="content">
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-
<div class="post">
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-
<h2 class="title">Welcome to ECHO,Tsinghua-A/PARTS</a></h2>
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<p></p>
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<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
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<HTML>
<HTML>
<HEAD>
<HEAD>
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<META NAME="CREATED" CONTENT="20111002;3132500">
<META NAME="CREATED" CONTENT="20111002;3132500">
<META NAME="CHANGEDBY" CONTENT="Sl Y">
<META NAME="CHANGEDBY" CONTENT="Sl Y">
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<META NAME="CHANGED" CONTENT="20111002;3152300">
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<META NAME="CHANGED" CONTENT="20111002;20522400">
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<META NAME="CHANGEDBY" CONTENT="Sl Y">
</HEAD>
</HEAD>
<BODY LANG="zh-CN" DIR="LTR">
<BODY LANG="zh-CN" DIR="LTR">
-
<P><BR><BR>
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<br><br>
 +
<div class="nav">
 +
<table id="toc" class="toc">
 +
<tr>
 +
<td>
 +
<div id="toctitle">
 +
<h2>Contents</h2>
 +
</div>
 +
<ul>
 +
<li class="toclevel-1 tocsection-1"><a href="#Parts List"><span class="tocnumber">1</span> <span class="toctext">Parts List</span></a></li>
 +
<li class="toclevel-1 tocsection-2"><a href="#Descriptions"><span class="tocnumber">2</span> <span class="toctext">Descriptions</span></a></li>
 +
<li class="toclevel-1 tocsection-3"><a href="#Methods"><span class="tocnumber">3</span> <span class="toctext">Methods</span></a></li>
 +
<li class="toclevel-1 tocsection-4"><a href="#Results"><span class="tocnumber">4</span> <span class="toctext">Results</span></a></li>
 +
</ul>
 +
</td>
 +
</tr>
 +
</table>
 +
</div>
 +
<br><!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
 +
<HTML>
 +
<HEAD>
 +
<META HTTP-EQUIV="CONTENT-TYPE" CONTENT="text/html; charset=gb2312">
 +
<TITLE></TITLE>
 +
<META NAME="GENERATOR" CONTENT="OpenOffice.org 3.2  (Win32)">
 +
<META NAME="AUTHOR" CONTENT="Sl Y">
 +
<META NAME="CREATED" CONTENT="20111028;10190100">
 +
<META NAME="CHANGEDBY" CONTENT="Sl Y">
 +
<META NAME="CHANGED" CONTENT="20111028;10224900">
 +
</HEAD>
 +
<BODY LANG="zh-CN" DIR="LTR">
 +
<H1 id="Parts List"><A NAME="Parts List"></A><FONT COLOR="#006b6b"><FONT FACE="Arial, sans-serif"><FONT SIZE=5 STYLE="font-size: 20pt"><SPAN LANG="en-US"><B>Parts
 +
List</B></SPAN></FONT></FONT></FONT></H1>
 +
<HR SIZE=1>
 +
<P STYLE="margin-bottom: 0cm; line-height: 0.58cm; widows: 2; orphans: 2">
 +
<BR>
</P>
</P>
-
<TABLE WIDTH=961 BORDER=0 CELLPADDING=0 CELLSPACING=0 BGCOLOR="#ccccff">
+
<CENTER>
-
<COL WIDTH=9>
+
<TABLE WIDTH=760 BORDER=4 CELLPADDING=0 CELLSPACING=0 BGCOLOR="#ffffff">
-
<COL WIDTH=8>
+
<COL WIDTH=160>
-
<COL WIDTH=210>
+
<COL WIDTH=130>
-
<COL WIDTH=224>
+
<COL WIDTH=340>
-
<COL WIDTH=247>
+
<COL WIDTH=130>
-
<COL WIDTH=98>
+
<TR>
-
<COL WIDTH=70>
+
<TH WIDTH=160 BGCOLOR="#aaaaff">
-
<COL WIDTH=95>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Name</SPAN></FONT></FONT></FONT></P>
-
<TR>
+
</TH>
-
<TH COLSPAN=2 WIDTH=17 BGCOLOR="#aaaaff">
+
<TH WIDTH=130 BGCOLOR="#aaaaff">
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><BR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Type</SPAN></FONT></FONT></FONT></P>
-
</P>
+
</TH>
-
</TH>
+
<TH WIDTH=340 BGCOLOR="#aaaaff">
-
<TH WIDTH=210 BGCOLOR="#aaaaff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Description</SPAN></FONT></FONT></FONT></P>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Name</SPAN></FONT></FONT></P>
+
</TH>
-
</TH>
+
<TH WIDTH=130 BGCOLOR="#aaaaff">
-
<TH WIDTH=224 BGCOLOR="#aaaaff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Length</SPAN></FONT></FONT></FONT></P>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Type</SPAN></FONT></FONT></P>
+
</TH>
-
</TH>
+
</TR>
-
<TH WIDTH=247 BGCOLOR="#aaaaff">
+
<TR>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Description</SPAN></FONT></FONT></P>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
</TH>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574000"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574000</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
<TH WIDTH=98 BGCOLOR="#aaaaff">
+
</TD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Designer</SPAN></FONT></FONT></P>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TH>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
<TH WIDTH=70 BGCOLOR="#aaaaff">
+
</TD>
-
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Length</SPAN></FONT></FONT></P>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
</TH>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pLux
-
<TH WIDTH=95 BGCOLOR="#aaaaff">
+
+ TetR</SPAN></FONT></FONT></FONT></P>
-
<P><BR>
+
</TD>
-
</P>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TH>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">903</SPAN></FONT></FONT></FONT></P>
-
</TR>
+
</TD>
-
<TR>
+
</TR>
-
<TD WIDTH=9 BGCOLOR="#ddddff">
+
<TR>
-
<P STYLE="border: none; padding: 0cm"><BR>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
</P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574001"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574001</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=8 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P STYLE="border: none; padding: 0cm"><BR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
</P>
+
</TD>
-
</TD>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<TD WIDTH=210 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">TetR
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574000"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5>BBa_K574000</FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
regulated by 3OC6HSL</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=224 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1890</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=247 BGCOLOR="#ddddff">
+
</TR>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">LuxR
+
<TR>
-
regulated TetR</SPAN></FONT></FONT></P>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574002"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574002</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
<TD WIDTH=98 BGCOLOR="#ddddff">
+
</TD>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Lei
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
Wei</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=70 BGCOLOR="#ddddff">
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<P ALIGN=RIGHT STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">903</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">pluxR
-
</TD>
+
+ CFP</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=95 BGCOLOR="#ddddff">
+
</TD>
-
<P><BR>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">941</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
</TR>
+
</TR>
-
<TR>
+
<TR>
-
<TD WIDTH=9 BGCOLOR="#ddddff">
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<P STYLE="border: none; padding: 0cm"><BR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574003"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574003</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
</P>
+
</TD>
-
</TD>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<TD WIDTH=8 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></FONT></P>
-
<P STYLE="border: none; padding: 0cm"><BR>
+
</TD>
-
</P>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC6HSL
-
<TD WIDTH=210 BGCOLOR="#ddddff">
+
-&gt; TetR &amp;&amp; reporter</SPAN></FONT></FONT></FONT></P>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574001"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5>BBa_K574001</FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
</TD>
-
</TD>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<TD WIDTH=224 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2839</SPAN></FONT></FONT></FONT></P>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
</TD>
-
</TD>
+
</TR>
-
<TD WIDTH=247 BGCOLOR="#ddddff">
+
<TR>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">TetR
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
regulated by 3OC6HSL</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574004"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574004</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=98 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Lei
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
Wei</SPAN></FONT></FONT></P>
+
</TD>
-
</TD>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<TD WIDTH=70 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL
-
<P ALIGN=RIGHT STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">1890</SPAN></FONT></FONT></P>
+
regulated by TetR</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=95 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P><BR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">797</SPAN></FONT></FONT></FONT></P>
-
</P>
+
</TD>
-
</TD>
+
</TR>
-
</TR>
+
<TR>
-
<TR>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<TD WIDTH=9 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574005"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574005</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
<P STYLE="border: none; padding: 0cm"><BR>
+
</TD>
-
</P>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=8 BGCOLOR="#ddddff">
+
</TD>
-
<P STYLE="border: none; padding: 0cm"><BR>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
</P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL
-
</TD>
+
and YFP regulated by pBad</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=210 BGCOLOR="#ddddff">
+
</TD>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574002"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5>BBa_K574002</FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1712</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=224 BGCOLOR="#ddddff">
+
</TD>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
</TR>
-
</TD>
+
<TR>
-
<TD WIDTH=247 BGCOLOR="#ddddff">
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">pluxR
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574006"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574006</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
+ CFP</SPAN></FONT></FONT></P>
+
</TD>
-
</TD>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<TD WIDTH=98 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Device</SPAN></FONT></FONT></FONT></P>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Lei
+
</TD>
-
Wei</SPAN></FONT></FONT></P>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL
-
<TD WIDTH=70 BGCOLOR="#ddddff">
+
regulated by pTet and pBad</SPAN></FONT></FONT></FONT></P>
-
<P ALIGN=RIGHT STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">941</SPAN></FONT></FONT></P>
+
</TD>
-
</TD>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<TD WIDTH=95 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">2517</SPAN></FONT></FONT></FONT></P>
-
<P><BR>
+
</TD>
-
</P>
+
</TR>
-
</TD>
+
<TR>
-
</TR>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<TR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574007"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574007</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
<TD WIDTH=9 BGCOLOR="#ddddff">
+
</TD>
-
<P STYLE="border: none; padding: 0cm"><BR>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=8 BGCOLOR="#ddddff">
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<P STYLE="border: none; padding: 0cm"><BR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">lasR
-
</P>
+
+ TT</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=210 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574003"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5>BBa_K574003</FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">918</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
</TD>
-
<TD WIDTH=224 BGCOLOR="#ddddff">
+
</TR>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P>
+
<TR>
-
</TD>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
<TD WIDTH=247 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574008"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574008</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Part
+
</TD>
-
1/3 of a Oscillator</SPAN></FONT></FONT></P>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=98 BGCOLOR="#ddddff">
+
</TD>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Lei
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
Wei</SPAN></FONT></FONT></P>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">a
-
</TD>
+
constitutive LasR producer</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=70 BGCOLOR="#ddddff">
+
</TD>
-
<P ALIGN=RIGHT STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">2839</SPAN></FONT></FONT></P>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
</TD>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">982</SPAN></FONT></FONT></FONT></P>
-
<TD WIDTH=95 BGCOLOR="#ddddff">
+
</TD>
-
<P><BR>
+
</TR>
-
</P>
+
<TR>
-
</TD>
+
<TD WIDTH=160 BGCOLOR="#ddddff">
-
</TR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">BBa_K574009</SPAN></FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
-
<TR>
+
</TD>
-
<TD WIDTH=9 BGCOLOR="#ddddff">
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<P STYLE="border: none; padding: 0cm"><BR>
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></FONT></P>
-
</P>
+
</TD>
-
</TD>
+
<TD WIDTH=340 BGCOLOR="#ddddff">
-
<TD WIDTH=8 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">3OC12HSL
-
<P STYLE="border: none; padding: 0cm"><BR>
+
-&gt; PoPS Receiver</SPAN></FONT></FONT></FONT></P>
-
</P>
+
</TD>
-
</TD>
+
<TD WIDTH=130 BGCOLOR="#ddddff">
-
<TD WIDTH=210 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="border: none; padding: 0cm"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=4><SPAN LANG="en-US">1147</SPAN></FONT></FONT></FONT></P>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574004"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5>BBa_K574004</FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
</TD>
-
</TD>
+
</TR>
-
<TD WIDTH=224 BGCOLOR="#ddddff">
+
</TABLE>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
</CENTER>
-
</TD>
+
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
-
<TD WIDTH=247 BGCOLOR="#ddddff">
+
</P>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">lasI
+
<H1 id="Descriptions" STYLE="page-break-before: always; page-break-after: avoid"><A NAME="Descriptions"></A>
-
regulated by p(TetR)</SPAN></FONT></FONT></P>
+
<FONT COLOR="#006b6b"><FONT FACE="Arial, sans-serif"><FONT SIZE=5 STYLE="font-size: 20pt"><SPAN LANG="en-US">Descriptions</SPAN></FONT></FONT></FONT></H1>
-
</TD>
+
<HR SIZE=1>
-
<TD WIDTH=98 BGCOLOR="#ddddff">
+
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Lei
+
</P>
-
Wei</SPAN></FONT></FONT></P>
+
<P STYLE="margin-left: 1.13cm; line-height: 200%; widows: 2; orphans: 2">
-
</TD>
+
<FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">We
-
<TD WIDTH=70 BGCOLOR="#ddddff">
+
assembled&nbsp;</SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_E0840"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_E0840</SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;under
-
<P ALIGN=RIGHT STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">797</SPAN></FONT></FONT></P>
+
the pLas promoter&nbsp;</SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_R0079"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_R0079</SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;that
-
</TD>
+
was contained into K574009. We kept pSB1A2 as the scaffold vector.</SPAN></SPAN></FONT></FONT></FONT></SPAN></FONT></P>
-
<TD WIDTH=95 BGCOLOR="#ddddff">
+
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
-
<P><BR>
+
</P>
-
</P>
+
<H1 id="Methods" STYLE="page-break-before: always; page-break-after: avoid"><A NAME="Methods"></A>
-
</TD>
+
<FONT COLOR="#006b6b"><FONT FACE="Arial, sans-serif"><FONT SIZE=5 STYLE="font-size: 20pt"><SPAN LANG="en-US">Methods</SPAN></FONT></FONT></FONT></H1>
-
</TR>
+
<HR SIZE=1>
-
<TR>
+
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
-
<TD WIDTH=9 BGCOLOR="#ddddff">
+
</P>
-
<P STYLE="border: none; padding: 0cm"><BR>
+
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
-
</P>
+
<FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">1.
-
</TD>
+
Ligate&nbsp;</SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_E0840"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_E0840</SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;downstream
-
<TD WIDTH=8 BGCOLOR="#ddddff">
+
of&nbsp;</SPAN></SPAN></FONT></FONT></FONT><A HREF="http://partsregistry.org/wiki/index.php/Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">BBa_K574009</SPAN></SPAN></FONT></FONT></SPAN></FONT></A><FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN STYLE="font-style: normal"><SPAN STYLE="font-weight: normal">&nbsp;,
-
<P STYLE="border: none; padding: 0cm"><BR>
+
which acts as a reporter.</SPAN></SPAN></FONT></FONT></FONT></SPAN></FONT></P>
-
</P>
+
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
-
</TD>
+
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">2.
-
<TD WIDTH=210 BGCOLOR="#ddddff">
+
Culture the positive colony at 37&deg;C, 220 rpm for 12 hours, as
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574005"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5>BBa_K574005</FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
+
well as the DH5alpha.</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
-
<TD WIDTH=224 BGCOLOR="#ddddff">
+
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">3.
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
+
Dilute 1:25 the former in 15 falcon tubes (4 ml cultures), each 3
-
</TD>
+
tubes as a group. The later is also diluted in 3 tubes as the
-
<TD WIDTH=247 BGCOLOR="#ddddff">
+
negative control.</SPAN></FONT></FONT></FONT></P>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">lasI
+
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
-
and YFP regulated by pBAD</SPAN></FONT></FONT></P>
+
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">4.
-
</TD>
+
After culturing for another 2 hours, as their OD600 reach 0.2, induce
-
<TD WIDTH=98 BGCOLOR="#ddddff">
+
with 3OC12HSL 0, 1e-7 M, 1e-6 M, 1e-5 M and 1e-4 M respectively.</SPAN></FONT></FONT></FONT></P>
-
<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Lei
+
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
-
Wei</SPAN></FONT></FONT></P>
+
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">5.
-
</TD>
+
Take samples after 1 hour, 4 hours, 8 hours and 24 hours, that is,
-
<TD WIDTH=70 BGCOLOR="#ddddff">
+
centrifugalizate 0.5 mL of each culture and suspend them by 1 mL of
-
<P ALIGN=RIGHT STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">1712</SPAN></FONT></FONT></P>
+
PBS (phosphate-buffered saline).</SPAN></FONT></FONT></FONT></P>
-
</TD>
+
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
-
<TD WIDTH=95 BGCOLOR="#ddddff">
+
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">6.
-
<P><BR>
+
Measure the fluorescence of the samples with the flow cytometer.</SPAN></FONT></FONT></FONT></P>
-
</P>
+
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
-
</TD>
+
</P>
-
</TR>
+
<H1 id="Results" STYLE="page-break-before: always; page-break-after: avoid"><A NAME="Results"></A>
-
<TR>
+
<FONT COLOR="#006b6b"><FONT FACE="Arial, sans-serif"><FONT SIZE=5 STYLE="font-size: 20pt"><SPAN LANG="en-US">Results</SPAN></FONT></FONT></FONT></H1>
-
<TD WIDTH=9 BGCOLOR="#ddddff">
+
<HR SIZE=1>
-
<P STYLE="border: none; padding: 0cm"><BR>
+
<P STYLE="line-height: 0.58cm; widows: 2; orphans: 2"><BR><BR>
-
</P>
+
</P>
-
</TD>
+
<P ALIGN=CENTER STYLE="line-height: 200%; widows: 2; orphans: 2"><FONT COLOR="#000000"><IMG SRC="https://static.igem.org/mediawiki/2011/c/ca/K574009_chart.png" NAME="K574009_chart" ALIGN=BOTTOM WIDTH=770 HEIGHT=490 BORDER=0></FONT></P>
-
<TD WIDTH=8 BGCOLOR="#ddddff">
+
<P ALIGN=CENTER STYLE="line-height: 200%; widows: 2; orphans: 2"><BR><BR>
-
<P STYLE="border: none; padding: 0cm"><BR>
+
</P>
-
</P>
+
<P STYLE="margin-left: 1.16cm; line-height: 200%; widows: 2; orphans: 2">
-
</TD>
+
<FONT COLOR="#000000"><FONT FACE="Arial, sans-serif"><FONT SIZE=3 STYLE="font-size: 12pt"><SPAN LANG="en-US">From
-
<TD WIDTH=210 BGCOLOR="#ddddff">
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the chart, we can see that cells were hardly induced in the control
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574006"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5>BBa_K574006</FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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group, and with the concentration of inducer growing, the intensity
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</TD>
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of GFP increased by groups. The most efficient concentration of
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<TD WIDTH=224 BGCOLOR="#ddddff">
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inducer was around 10<SUP>-5</SUP>M, and higher concentration may
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Device</SPAN></FONT></FONT></P>
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lead to the expression of GFP decreasing. Additionally, to most
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groups, the intensity of GFP reached its maximum after 4 hours.</SPAN></FONT></FONT></FONT></P>
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<P><FONT FACE="Arial, sans-serif"><SPAN LANG="en-US"><BR></SPAN></FONT><BR><BR>
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Part
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2/3 of a Oscillator</SPAN></FONT></FONT></P>
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<TD WIDTH=98 BGCOLOR="#ddddff">
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Lei
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Wei</SPAN></FONT></FONT></P>
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<TD WIDTH=70 BGCOLOR="#ddddff">
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<P ALIGN=RIGHT STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">2517</SPAN></FONT></FONT></P>
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<TD WIDTH=95 BGCOLOR="#ddddff">
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<TD WIDTH=210 BGCOLOR="#ddddff">
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574007"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5>BBa_K574007</FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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</TD>
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<TD WIDTH=224 BGCOLOR="#ddddff">
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
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<TD WIDTH=247 BGCOLOR="#ddddff">
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">lasR
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Lei
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Wei</SPAN></FONT></FONT></P>
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</TD>
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<P ALIGN=RIGHT STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">918</SPAN></FONT></FONT></P>
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574008"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5>BBa_K574008</FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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</TD>
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<TD WIDTH=224 BGCOLOR="#ddddff">
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
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<TD WIDTH=247 BGCOLOR="#ddddff">
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">const
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lasR</SPAN></FONT></FONT></P>
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<TD WIDTH=98 BGCOLOR="#ddddff">
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Lei
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Wei</SPAN></FONT></FONT></P>
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</TD>
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<TD WIDTH=70 BGCOLOR="#ddddff">
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<P ALIGN=RIGHT STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">982</SPAN></FONT></FONT></P>
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="http://partsregistry.org/wiki/index.php?title=Part:BBa_K574009"><FONT COLOR="#002bb8"><SPAN STYLE="text-decoration: none"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5>BBa_K574009</FONT></FONT></SPAN></FONT></A></SPAN></FONT></P>
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Composite</SPAN></FONT></FONT></P>
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">PoPS
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-&gt; LasI</SPAN></FONT></FONT></P>
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<TD WIDTH=98 BGCOLOR="#ddddff">
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<P STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">Lei
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<P ALIGN=RIGHT STYLE="border: none; padding: 0cm"><FONT FACE="Arial, Helvitica, sans-serif"><FONT SIZE=5><SPAN LANG="en-US">1147</SPAN></FONT></FONT></P>
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<P><FONT FACE="Times New Roman, serif"><SPAN LANG="en-US"><A HREF="https://static.igem.org/mediawiki/2011/0/0b/Ref.pdf">Click
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to download pdf</A></SPAN></FONT></P>
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Latest revision as of 02:28, 28 October 2011

JavaScript Slideshow - TinySlideshow



Contents

Parts List


Name

Type

Description

Length

BBa_K574000

Composite

pLux + TetR

903

BBa_K574001

Composite

TetR regulated by 3OC6HSL

1890

BBa_K574002

Composite

pluxR + CFP

941

BBa_K574003

Device

3OC6HSL -> TetR && reporter

2839

BBa_K574004

Composite

3OC12HSL regulated by TetR

797

BBa_K574005

Composite

3OC12HSL and YFP regulated by pBad

1712

BBa_K574006

Device

3OC12HSL regulated by pTet and pBad

2517

BBa_K574007

Composite

lasR + TT

918

BBa_K574008

Composite

a constitutive LasR producer

982

BBa_K574009

Composite

3OC12HSL -> PoPS Receiver

1147



Descriptions



We assembled BBa_E0840 under the pLas promoter BBa_R0079 that was contained into K574009. We kept pSB1A2 as the scaffold vector.



Methods



1. Ligate BBa_E0840 downstream of BBa_K574009 , which acts as a reporter.

2. Culture the positive colony at 37°C, 220 rpm for 12 hours, as well as the DH5alpha.

3. Dilute 1:25 the former in 15 falcon tubes (4 ml cultures), each 3 tubes as a group. The later is also diluted in 3 tubes as the negative control.

4. After culturing for another 2 hours, as their OD600 reach 0.2, induce with 3OC12HSL 0, 1e-7 M, 1e-6 M, 1e-5 M and 1e-4 M respectively.

5. Take samples after 1 hour, 4 hours, 8 hours and 24 hours, that is, centrifugalizate 0.5 mL of each culture and suspend them by 1 mL of PBS (phosphate-buffered saline).

6. Measure the fluorescence of the samples with the flow cytometer.



Results





From the chart, we can see that cells were hardly induced in the control group, and with the concentration of inducer growing, the intensity of GFP increased by groups. The most efficient concentration of inducer was around 10-5M, and higher concentration may lead to the expression of GFP decreasing. Additionally, to most groups, the intensity of GFP reached its maximum after 4 hours.




Retrieved from "http://2011.igem.org/Team:Tsinghua-A/Parts"