Team:uOttawa

From 2011.igem.org

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             <a href="/Team:uOttawa/Project"><img border="0" src="https://static.igem.org/mediawiki/2011/5/53/Uo_project-overview.png" alt="Sponsors" /></a>
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             <a href="/Team:uOttawa/Results"><img border="0" src="https://static.igem.org/mediawiki/2011/8/8a/Uo_results.png" alt="Sponsors" /></a>
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             <a href="/Team:uOttawa/Parts"><img border="0" src="https://static.igem.org/mediawiki/2011/f/f4/Uo_parts-submitted.png" alt="Sponsors" /></a>
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             <a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=uOttawa" target="_blank"><img border="0" src="https://static.igem.org/mediawiki/2011/f/f4/Uo_parts-submitted.png" alt="Sponsors" /></a>
             <a href="/Team:uOttawa/Game"><img border="0" src="https://static.igem.org/mediawiki/2011/0/0c/Uo_gears-of-evolution.png" alt="Sponsors" /></a>
             <a href="/Team:uOttawa/Game"><img border="0" src="https://static.igem.org/mediawiki/2011/0/0c/Uo_gears-of-evolution.png" alt="Sponsors" /></a>
             <a href="/Team:uOttawa/Sponsors"><img border="0" src="https://static.igem.org/mediawiki/2011/7/7d/Uo_sponsors.png" alt="Sponsors" /></a>
             <a href="/Team:uOttawa/Sponsors"><img border="0" src="https://static.igem.org/mediawiki/2011/7/7d/Uo_sponsors.png" alt="Sponsors" /></a>
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<p>Yeast has become a model organism due to its homology with mammalian organisms and its ease of genetic manipulation. For this reason, we believe it is important to develop tools that improve our ability to use yeast as a standard BioBrick organism. To this end, we aim to submit several BioBricks optimized to work in the simple eukaryote S. cerevisiae as well as build a novel yeast chassis that is capable of the robust characterization of these Biobricks. In addition, we are developing a new assembly protocol that will greatly improve upon existing BioBrick assembly protocols and allow for rapid integration into S. cerevisiae.</p>
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<p> <i>Saccharomyces cerevisiae</i> (budding or brewer's yeast) has emerged as an important micro-organism in biomedical research and industry. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in <i>S. cerevisiae</i>. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have also submitted several BioBricks optimized for use in <i>S. cerevisiae</i>. Finally, we succeeded in developing BrickMason Assembly, a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols. So raise a glass to your favourite single-celled eukaryote, and let yeast leaven your spirits.</p>
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<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/8/88/Team_uo.png') no-repeat !important;"><span>Team</span></h2></html><p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Cras non orci leo, et feugiat neque. Proin orci magna, faucibus eu consectetur euismod, malesuada ac eros. Mauris vestibulum, lectus eleifend egestas condimentum, nibh magna molestie lectus, eu varius dolor ipsum ac velit. Cras et tincidunt nibh. Sed elementum turpis eget nulla dapibus luctus. Maecenas tempus mi eu sapien consectetur tempus. Donec gravida felis in purus rutrum at tincidunt metus commodo.</p>
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<html><a href="/Team:uOttawa/Team"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/8/88/Team_uo.png') no-repeat !important;"><span>Team</span></h2></a></html><p>This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.</p>
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<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/b/b9/Project_uo.png') no-repeat !important;"><span>Project</span></h2></html><p>Duis consequat tortor vitae ipsum suscipit et sodales enim aliquet. Duis varius mi egestas nibh dictum eget tristique nibh iaculis. Aliquam erat volutpat. Pellentesque auctor, lectus vitae sodales vestibulum, odio ante scelerisque ante, auctor pellentesque felis odio ac turpis.</p>
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<html><a href="/Team:uOttawa/NewAssemblyProtocol"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/c/cd/BM3.png') no-repeat !important;"><span>BrickMason Assembly</span></h2></a></html><p>Here we describe a convenient, efficient and cost-effective assembly method known as BrickMason assembly. Click on the image above to check out our animation and see how it works!</p>
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<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/5/51/Results_uo.png') no-repeat !important;"><span>Results</span></h2></html><p>Text here.</p>
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<html><a href="/Team:uOttawa/Results"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/5/51/Results_uo.png') no-repeat !important;"><span>Results</span></h2></a></html><p>Here we successfully demonstrate that BrickMason assembly can be used to assemble 6 brick constructs in just one days work. In addition, we were able to characterize 3 new BioBricks.</p>
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<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/2/2d/Parts_uo.png') no-repeat !important;"><span>Parts Submitted</span></h2></html><p>Heck yeah.</p>
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<html><a target="_blank" href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2011&group=uOttawa"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/2/2d/Parts_uo.png') no-repeat !important;"><span>Parts Submitted</span></h2></a></html><p>We submitted 8 new BioBricks to the registry this year.</p>
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<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/0/0d/Human_uo.png') no-repeat !important;"><span>Human Practices</span></h2></html><p>Text here.</p>
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<html><a href="/Team:uOttawa/Game"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/0/0d/Human_uo.png') no-repeat !important;"><span>Gears of Evolution</span></h2></a></html><p>The uOttawa team has designed a 10 level game intended to teach and inform high school students about synthetic biology. This game is fully playable on our wiki, the iGEM community page and the Openwetware site Community Bricks. Click on the image above to play it and please give us feedback!</p>
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<html><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/e/e5/Protocols_uo.png') no-repeat !important;"><span>Protocols/Milestones</span></h2></html><p>Text here.</p>
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<html><a href="/Team:uOttawa/Sponsors"><h2 class="title" style="background: url('https://static.igem.org/mediawiki/2011/1/1f/Sponsors_uo.png') no-repeat !important;"><span>Sponsors</span></h2></a></html><p>The uOttawa team would like to thank our sponsors for their generous support this year. Our sponsors graciously supplied us with lab equipment, reagents and funds necessary for the team to compete.</p>
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{{uOttawa_Footer}}
{{uOttawa_Footer}}

Latest revision as of 07:52, 27 October 2011


Sponsors Sponsors Sponsors Sponsors Sponsors Sponsors
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Saccharomyces cerevisiae (budding or brewer's yeast) has emerged as an important micro-organism in biomedical research and industry. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in S. cerevisiae. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have also submitted several BioBricks optimized for use in S. cerevisiae. Finally, we succeeded in developing BrickMason Assembly, a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols. So raise a glass to your favourite single-celled eukaryote, and let yeast leaven your spirits.

Team

This year’s team brought together many students from different faculties. With fundraising, computer programming, website design and wet lab work there was a role for everyone.

BrickMason Assembly

Here we describe a convenient, efficient and cost-effective assembly method known as BrickMason assembly. Click on the image above to check out our animation and see how it works!

Results

Here we successfully demonstrate that BrickMason assembly can be used to assemble 6 brick constructs in just one days work. In addition, we were able to characterize 3 new BioBricks.

Parts Submitted

We submitted 8 new BioBricks to the registry this year.

Gears of Evolution

The uOttawa team has designed a 10 level game intended to teach and inform high school students about synthetic biology. This game is fully playable on our wiki, the iGEM community page and the Openwetware site Community Bricks. Click on the image above to play it and please give us feedback!

Sponsors

The uOttawa team would like to thank our sponsors for their generous support this year. Our sponsors graciously supplied us with lab equipment, reagents and funds necessary for the team to compete.