Team:Peking S

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  <p align="center"><font face="Castellar" class="ws20"><B>PROJECT DESCRIPTION</B></font></p>
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<font face="verdana "class="ws14" color="#ffffff">TO IGEM HQ!
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Cell-cell communication-based multicellular networks provide an extended vista for synthetic biology. By compartmentalizing complex genetic circuits into separate engineered cells, the difficulty of the construction by layering elementary logic gates can be dramatically reduced, partly due to the insulation of crosstalk between modules, the suppression of noise by populationally averaging, and the reducing of metabolic burden in host cells. What’s more, cell-cell communication-based multicellular feature also enables coordination and synchronization among cells in and between populations and facilitates the generation of reliable non-Boolean dynamics.
 
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However, orthogonal ‘chemical wires’ are far from sufficient to allow concurrent communication. Accordingly, our project intends to develop a versatile ‘chemical wire’ toolkit for both multicellular Boolean computing and non-Boolean dynamics. Criteria and assays for ‘chemical wire’ characterization and validation have been established, in which the orthogonality, the speed of signal relaying and the ability to coordinate cells are verified. A set of recently reported quorum sensing systems have been selected, together with previously investigated AHL system, as ‘chemical wire’ toolbox. With this toolbox, multicellular design principles for complex logic circuit that allow a reduction in the complexity and a guarantee in both robust function and operation speed of a system will be proposed, following which reusable E.coli cell types that each compartmentalizes specific elementary logic gate in a single cell, will be constructed, connected by ‘chemical wires’. By layering a very few number of such cellular logic gates, complex logic computing process will be easily implemented. Besides, a tunable queen-worker-interaction based cell density oscillator will be constructed, in order to verify the feasibility of our ‘chemical wire’ toolkit for non-Boolean dynamic functions. Nonetheless, challenges remain for this non-Boolean process because communication signals are produced by growing cells, i.e. changes in cell densities may affect the strength of communication. To crack these hurdles, we will develop a microfluidic device that imposes constraints on cell densities and growth condition to guarantee well-coordinated population dynamics.
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<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>A </span><span style="font-size:16px"><font color="#ffffff" class="ws16">&#8216;Chemical Wire&#8217; Toolbox for Synthetic Microbial Consortia</B></font></span></div>
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<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Q</span><span style="font-size:16px"><font color="#ffffff" class="ws16">uick Links</B></font></span></div>
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<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>S</span><span style="font-size:16px"><font color="#ffffff" class="ws16">ponsed By</B></font></span></div>
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<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Click below</span><span style="font-size:16px"><font color="#000000" class="ws16"> To access a brief introduction of our project<br><br></B></font></span></div>
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  <a href="https://2011.igem.org/Team:Peking_S/lab/biosafety" class="project"><font=verdana><span class="project">GO TO SAFETY PAGE</span></a></p><p class="mainbody">&nbsp;</p></th>
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<b>Project presentation</b>
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<b>Cell-cell communication</b>-based multicellular networks provide an extended vista for synthetic biology. However, ‘chemical wires’ that allow versatile concurrent communications are far from sufficient.
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Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression systems have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density has been created with supporting microfluid device.
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<a href=https://2011.igem.org/Team:Peking_S/project>
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<div id="image1" style="position:absolute; overflow:hidden; left:489px; top:1134px; width:292px; height:37px; z-index:40"><a href="http://www.phy.pku.edu.cn/English.html"><img src="https://static.igem.org/mediawiki/2011/7/7e/Pkuphy1.png"  alt="" title="" border=0 width=292 height=37></div>
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<div id="image2" style="position:absolute; overflow:hidden; left:501px; top:1230px; width:294px; height:36px; z-index:41"><a href="http://ctb.pku.edu.cn/main/en/index.htm"><img src="https://static.igem.org/mediawiki/2011/8/8e/PekingR_PKU_CTB.gif" alt="" title="" border=0 width=294 height=36></div>
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<div id="image3" style="position:absolute; overflow:hidden; left:697px; top:1182px; width:194px; height:43px; z-index:42"><a href="http://dean.pku.edu.cn/"><img src="https://static.igem.org/mediawiki/2011/2/20/PekingR_PKU_OFFICE_OF_EDUCATIONAL_ADMINISTRATION_%281%29.png" alt="" title="" border=0 width=194 height=43></div>
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<div id="image8" style="position:absolute; overflow:hidden; left:472px; top:1325px; width:250px; height:35px; z-index:46"><a href="http://web5.pku.edu.cn/huanjing/"><img src="https://static.igem.org/mediawiki/2011/0/02/PekingR_COLLEGE_OF_ENVIRONMENTAL_SCIENCES_AND_ENGINEERING_%281%29.png" alt="" title="" border=0 width=250 height=35></div>
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Latest revision as of 05:31, 23 October 2011

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TO IGEM HQ!
A ‘Chemical Wire’ Toolbox for Synthetic Microbial Consortia
Quick Links
Sponsed By
Click below To access a brief introduction of our project

Project presentation
Cell-cell communication-based multicellular networks provide an extended vista for synthetic biology. However, ‘chemical wires’ that allow versatile concurrent communications are far from sufficient. Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression systems have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density has been created with supporting microfluid device.
learn more

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