Team:Peking S

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A ‘Chemical Wire’ Toolbox for Synthetic Microbial Consortia

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Sponsed By

Click below To access a brief introduction of our project

Project presentation
Cell-cell communication-based multicellular networks provide an extended vista for synthetic biology. However, ‘chemical wires’ that allow versatile concurrent communications are far from sufficient. Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression systems have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density has been created with supporting microfluid device.
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+<font face="verdana "class="ws14" color="#ffffff">TO IGEM HQ!
+<a href=https://2011.igem.org>
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+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>A </span><span style="font-size:16px"><font color="#ffffff" class="ws16">&#8216;Chemical Wire&#8217; Toolbox for Synthetic Microbial Consortia</B></font></span></div>
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+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Q</span><span style="font-size:16px"><font color="#ffffff" class="ws16">uick Links</B></font></span></div>
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-'''PROJECT SUMMARY'''
-There are many hindrances in construction of complex gene circuits, such as crosstalk between cellular elements, noise cascade as the increase of network complexity, and metabolic burden. Distributing complex gene circuits into different cells is one of valid methods to solve those problem. Among those, the most significant problem is the shortage of cell-cell communication systems. In order to solve that, we characterize two kind of cell-cell communication molecules .One of them is (S)-3-hydroxytridecan-4-one which is synthesized by Vibrio cholerae, and another is 2-isocapryloyl-3R-hydroxymethyl-g-butyrolactone synthesized by Streptomyces griseus . And AND , NOR , XOR gates will be constructed to prove their capabilities in building cell-cell communication system. As a result, we achieve to use one kind of cell to control the ratio of two kind of cells living together.
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+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>S</span><span style="font-size:16px"><font color="#ffffff" class="ws16">ponsed By</B></font></span></div>
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+<font color="#FFFF00" face="verdana" ><span style="font-size:21px"><B>Click below</span><span style="font-size:16px"><font color="#000000" class="ws16"> To access a brief introduction of our project<br><br></B></font></span></div>
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+<b>Project presentation</b>
+<br>
+</a>
+<b>Cell-cell communication</b>-based multicellular networks provide an extended vista for synthetic biology. However, ‘chemical wires’ that allow versatile concurrent communications are far from sufficient.
+Accordingly, our project intends to develop a versatile ‘chemical wire’ toolbox for both multicellular Boolean computing and non-Boolean dynamics by two approaches. Firstly, a set of recently reported novel quorum sensing systems have been characterized. Secondly, quorum sensing (QS) based transcriptional repression systems have been built from the ground up by conversing LuxR family of transcription activators into repressors. We next sought to develop design rules of microbial consortia as supplements to this toolbox. To validate this toolbox together with the design rules, several robust combinational and sequential logic circuits that are difficult to be implemented in single cell have been constructed as a proof of concept for Boolean logic. As for non-Boolean dynamics, a balancer of microbial population density has been created with supporting microfluid device.
+<br>
+<a href=https://2011.igem.org/Team:Peking_S/project>
+learn more
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+<div id="image3" style="position:absolute; overflow:hidden; left:697px; top:1182px; width:194px; height:43px; z-index:42"><a href="http://dean.pku.edu.cn/"><img src="https://static.igem.org/mediawiki/2011/2/20/PekingR_PKU_OFFICE_OF_EDUCATIONAL_ADMINISTRATION_%281%29.png" alt="" title="" border=0 width=194 height=43></div>
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+<div id="image8" style="position:absolute; overflow:hidden; left:472px; top:1325px; width:250px; height:35px; z-index:46"><a href="http://web5.pku.edu.cn/huanjing/"><img src="https://static.igem.org/mediawiki/2011/0/02/PekingR_COLLEGE_OF_ENVIRONMENTAL_SCIENCES_AND_ENGINEERING_%281%29.png" alt="" title="" border=0 width=250 height=35></div>
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