Team:UC Davis/Notebook wip

From 2011.igem.org

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== Week 1==
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-Rehydrated parts from 2011 Registry Distribution:
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**-R0040=Tet promoter in psb1a2
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**-C0040=Tet repressor in psb1a2
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**-C0012=LacI repressor in psb1a2
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**-C0051=Lambda cI repressor in psb1a2
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**-R0051=cI-regulated promoter in psb1a2
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**-I732006=LacZ-alpha in psb1ak3
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**-R0010=LacI regulated promoter in psb1a2
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**-E0040=GFP coding region in psb1a2
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**-J23101=Constitutive promoter in j61002
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**-C0080=AraC repressor/activator in psb2k3
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**-I13458=pC+AraC in psb1a3
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**-I13453=pBAD promoter in psb1a3
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**-B0015=Double terminator in psb1ak3
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-Transformed all hydrated parts
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-Cultured all parts and digested to check on a gel.  All parts checked out.  Sent out all hydrated parts to get sequenced.
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-Ligated mutants into screening plasmid to see if our error-prone PCRs had visible mutants.
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-Ordered primers for mutagenesis of LacI promoter, cI promoter and tet promoter.  Also ordered primers to replace RBS (Bba_B0032) in Bba_E0240 to Bba_B0034.   
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== Week 2==
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== Week 3 ==
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== Week 4 ==
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== Week 5 ==
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== Week 6 ==
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== Week 7 ==
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== Week 8 ==
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== Week 9 ==
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== Week 10 ==
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Revision as of 18:59, 24 June 2011

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== Week 1== -Rehydrated parts from 2011 Registry Distribution: **-R0040=Tet promoter in psb1a2 **-C0040=Tet repressor in psb1a2 **-C0012=LacI repressor in psb1a2 **-C0051=Lambda cI repressor in psb1a2 **-R0051=cI-regulated promoter in psb1a2 **-I732006=LacZ-alpha in psb1ak3 **-R0010=LacI regulated promoter in psb1a2 **-E0040=GFP coding region in psb1a2 **-J23101=Constitutive promoter in j61002 **-C0080=AraC repressor/activator in psb2k3 **-I13458=pC+AraC in psb1a3 **-I13453=pBAD promoter in psb1a3 **-B0015=Double terminator in psb1ak3 -Transformed all hydrated parts -Cultured all parts and digested to check on a gel. All parts checked out. Sent out all hydrated parts to get sequenced. -Ligated mutants into screening plasmid to see if our error-prone PCRs had visible mutants. -Ordered primers for mutagenesis of LacI promoter, cI promoter and tet promoter. Also ordered primers to replace RBS (Bba_B0032) in Bba_E0240 to Bba_B0034. == Week 2== == Week 3 == == Week 4 == == Week 5 == == Week 6 == == Week 7 == == Week 8 == == Week 9 == == Week 10 ==

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