Team:Freiburg/Notebook/1 August

From 2011.igem.org

(Difference between revisions)
(3.Transformation)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Freiburg/Templates/header}}
{{:Team:Freiburg/Templates/header}}
 +
<html>
 +
<div id="notebook-page-header">
 +
<div id="notebook-back" width="100px" >
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/29_July">Previous entry</a>
 +
</div>
 +
<div id="notebook-title">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 1 August </a>
 +
</div>
 +
<div id="notebook-next">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/2_August">Next entry</a>
 +
</div>
 +
</div>
 +
</html>
 +
==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
Line 118: Line 132:
<br>
<br>
-
'''Digestion of 3A Assembly'''
+
===='''Digestion of 3A Assembly'''====
Line 210: Line 224:
<br/>
<br/>
-
'''Ligation'''
+
===='''Ligation'''====
Line 302: Line 316:
<br/>
<br/>
-
'''Transformation'''
+
===='''Transformation'''====
Line 338: Line 352:
{| style="border-spacing:0;"
{| style="border-spacing:0;"
-
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| New Composite Part in pSB1T3 Vector with modified K124017 containing a strong RBS in front of first ATH
+
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| New Composite Part in pSB1T3 Vector with modified K124017 containing a strong RBS in front of first ATG
 +
RESULT: No colonies!!! :( Probably because of the Tet vector, since other members of the group also had problems. We are trying it again with the pSB1C3 Vector
|}
|}
Line 347: Line 362:
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
-
'''PCR'''
+
===PCR===
Line 415: Line 430:
How did you label the PCR-Product, where is it stored and what do you do next?
How did you label the PCR-Product, where is it stored and what do you do next?
 +
 +
 +
===3A-assembly===
 +
====1.Digestion====
 +
 +
'''Investigators: Theo'''
 +
 +
Digestion of PCR product.
 +
 +
*S39
 +
*S43
 +
 +
were digested with EcoRI and SpeI
 +
 +
*P18
 +
*P19
 +
*P20
 +
 +
were digested with XbaI and PstI.
 +
 +
 +
====2.Ligation====
 +
 +
'''Investigators: Theo'''
 +
 +
Ligation of:
 +
 +
*S39+P18
 +
*S43+P18
 +
*S39+P19
 +
*S43+P19
 +
*S39+P20
 +
*S43+P20
 +
 +
stored in Theo´s box.

Latest revision as of 01:03, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!