"

From 2011.igem.org

pET15b OmpA-GFP construct

 Colony PCR
 Tip a tiny bit of the colonies, dip some on a new LB plate and dive the others into PCR reaction mix.
 Chose 20 clonies, 13 are correct.
 Inoculate 5 of the correct ones in 5ml LB Amp+
 -YX

pet15b OmpA-HIV cleavege site-sh3

 Digest OmpA-HIV cleavege site-sh3 with BamHI&NdeI
 gel extraction