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From 2011.igem.org

pSB1C3

  mini pre. 4 tubes

Pro-rich mcherry parts

 freeze 1 tube
 1ml for sequencing
 mini pre.
 gel analysis(below)

p15A parts

 3 clones
 freeze 1 tube for each one
 1ml for sequencing
 mini pre.

PAA parts Em7-OHS parts Tar parts

 culture PCR
 one of PAA is correct