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From 2011.igem.org

OmpA-GFP expression 1. Small Test(Competent Cell is BL21ODE) Group 1 2 Volume 3ml Pre-incubation(37℃,250rpm) 7h IPTG concentration(mM) 0

                                              0.1

0.5 1 IPTG induced temperature 30℃ 18℃ Duration 7h 12h Description Medium remained yellow Medium slightly changed to green E.coli white E.coli Green florescence

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