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Team:Toronto/Notebook/8 June 2011
From 2011.igem.org
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Matt
Training
E1010 samples where cultured overnight. There were 4 samples in all. 2 cultures were started from colony 1 while 1 culture each was started from colony 2 and 3
The overnight cultures were mini-prepped using a QIAprep Spin Mini prep Kit Concentratons were determined by Nano-drop (noted below) and placed on ice fo down stream applications.
Sample Concentrations
- 33.7 ng/uL
- 39.5 ng/uL
- 38.8 ng/uL
- 39.3 ng/uL
Samples were then digested with EcoRI and PstI to confirm E1010 was successfully obtained. Samples were digested at 37C for 1 hour and heat inactivated at 80C for 20 minutes.
500ng of DNA was digested
Digests were then ligated with T4 Ligase at RT overnight.
