-Speeding Up-

PCR HNS and electrophored for confirmation, but could not confirmated...

-Targetting-

@made culture media 5 plates (Amp)

@restriction enzyme
J23100→EcoRⅠ and SpeⅠ
p0440→EcoRⅠ and XbaⅠ
K317037→EcoRⅠ and XbaⅠ