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Team:Peking S/modeling/Parameters
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Population Density Balancer
Parameters
There are over 30 parameters in our model, which increases the difficulty of modeling conduction. This is similar to what we encountered in 2009 iGEM competition. Therefore we adopt some assumptions from 2009 Peking iGEM modeling work. According to those assumptions, we can decide either the value or the range of the parameters. Nevertheless, parameter c1 and c2 were not included, and we do not know their estimated value. Therefore, we sampled these two parameters in a quite wide range ( ) to guarantee that the appropriate value lies within sampling range.
To verify the credibility of the parameters values, we analyzed the sensitivity of parameters by following definition (the variation range of A cell population in steady state was employed to indicate the performance of the balancer): Each parameter varied respectively while other parameters fixed, then the extent of system performance variation was observed to calculate the sensitivity of each parameter.
The smaller the sensitivity value is, the more reasonable the parameters are.
Here we provide a graph to show the effect of parameter variation on the cell population dynamics.
Figure 2 the numerical simulation under different value of parameters. The red line is the simulation result of population of A under the value of parameters described in chart. The blue line denotes the result after increasing the value of alpha1 by 50%. And the yellow line represents the result after decreasing the value of alpha1 by 50%. The Figure demonstrates that despite of the change of alpha1, the dynamic property of population of A cell barely change. Meanwhile, the population of A cell at the steady state changes moderately compared with the change of alpha1. These phenomena imply that the parameters are not sensitive. (We got similar result when changing the value of other parameters whose simulation results are not shown here)
From the result of sensitivity analysis, we found that the sensitivity of our parameters is relatively low, while some of them are still sensitive enough, capable of tuning the proportion of A/B cell in the total population.
