Team:Peking R/Notebook/Protocols

From 2011.igem.org

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    Protocols of Experiments Ver. 2011  

DNA digestion protocol
DNA Gel extraction protocol
Miniprep Protocol
Protocol for chemical inducible expression of GFP
Protocol for ligation of insert DNA into plasmid vector DNA
Protocol for PCR with EasyPfu DNA Polymerase
Protocol for preparation of competent cells for transformation
Site-directed Mutagenesis protocol
Transformation protocol
Protocol for DNA purification from reaction mixture
Protocol for bistable switch characterization
Protocol for induce the AND gate
Protocol for ligand inducible expression of GFP
Protocol for selection

 

 

Protocols of Experiments Ver. 2010

SDS-PAGE & western blot protocol
SDS-PAGE & western sample preparation protocol
Protocol for chemical inducible expression of MBP & sample preparation
DNA Double Digestion Protocol
DNA Gel Extraction Protocol
Miniprep Protocol
Protocol for Chemical Inducible Expression of GFP
Protocol for DNA Purification from Reaction Mixture
Protocol for Ligation of Insert DNA into Plasmid Vector DNA
Protocol for PCR with EasyPfu DNA Polymerase
Protocol for Preparation of Competent Cells for Transformation
Site-directed Mutagenesis Protocol
Transformation Protocol
 
Protocols of Experiments Ver. 2009

Miniprep Protocol
DNA Gel Extraction Protocol
DNA Double Digestion Protocol
Protocol for DNA Purification From Reaction Mixture
Ligation of Inserting DNA into Plasmid Vector DNA
Protocol for PCR with EasyPfu DNA Polymerase
Protocol for preparation of competent cells for transformation
Site-directed mutagenesis protocol
Transformation Protocol
Protocol for Chemical Inducible Expression of GFP
   
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