Team:Paris Liliane Bettencourt/Notebook/2011/09/22/

From 2011.igem.org

Cyrille

YFP cloning

6 colonies from the ligation plate were taken and miniprep overday. The tube where prepared for the sequencing.

As there where very few µL left in the miniprep, a tranformation was done again and plated.

YFP QCM

The PCR product was digested one hour using fast digest DpnI. A gel was runned.

Then, the product was transformed. As I was not sure for one pipeting, two tubes where done for the 5ng plate. The result is plated and growth overnight.