Team:Paris Liliane Bettencourt/Notebook/2011/08/28/
From 2011.igem.org
Camille
From the culture that appeared to be good by the PCR colonies I did two minipreps.
Strain | [DNA] in ng/µL |
S88.1 | 234.7 |
S88.2 | 205.6 |
Then I digested it with Ecor1 and Xba1. I ligated it with pHyperspank as an insert, and transformed it in coli, I also transformed the overnight ligation from yesterday.