Team:Paris Liliane Bettencourt/Notebook/2011/07/20/

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    • T7 polymerase diffusion
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Mathias & Laura

T7 diffusion

Miniprep

-RFP Standard Terminator & RBS

-GFP mut3b T7 Terminator & RBS

-T7 K145001

Digestion and gel extraction

-RFP Standard Terminator & RBS by Xba1 and Pst1

-GFP mut3b T7 Terminator & RBS by Xba1 and Pst1

-T7 by Spe1 and Pst1

Ligation and transformation

-RFP Standard Terminator RBS & T7

-GFP mut3b T7 Terminator RBS & T7