Team:Paris Liliane Bettencourt/Notebook/2011/07/10/

From 2011.igem.org

Team IGEM Paris 2011

Contents

Mathias & Laura

Miniprep

-pVeg + SpoVg and LacI + double Terminator

-pHyperSpank + SpoVG K143055

Digestion and gel extraction

-pVeg + SpoVg and LacI + Term by EcoR1 and Spe1 

-pHyperSpank + SpoVg by EcoR1 and Xba1

Ligation and transformation

-pVeg + SpoVg LacI + Term & pHyperSpank + SpoVg  => LacI Expression System K606054