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From 2011.igem.org

We take a sample of rh1AB purifying gen without mutation and doing a PCR because doesn’t have a lot and we have to digest with Pst1. On Monday we are going to do a mutagenesis.

We use the PCR protocol that functions well with our gene (PCR Protocol) and we test all primers because we don’t know which primers the team used the last year for genes amplification.

Primers:

1. F:RhTf2a R:RhTR2a

2. F:RhT-f1b R:RhT-2b

3. F:RhTBio-R2a R:RhTBio-F1a

4. F:RhTBio-f1 R:RhTBio-2b

Meanwhile Orlando and Paul make the miniprep of (RBS + P) and (GFP + T) using the protocol of dirty miniprep on page 80.Right Now Sergio and Natasha take the miniprep results and check the concentration with Nanodrop while Paul prepares PCR.

RESULTS:

We have good levels of absorbance and concentration in the samples, we hope that the electrophoresis be good.

Link title

In sample #5 observe that the gene has a slightly amplification. Check pag 108 and 109 with Lorena because someone changes the PCR program. The gene doesn’t show any amplification. We have to make a PCR of the miniprep samples of the parts.