Team:Fatih Turkey/Notebook6

From 2011.igem.org

deneme baslik

Week:

21.08.11



Can=I742123
sht=K090403
K=J04450

We tried to different amount for  addition up, down, destination  part plasmid  in ligation protocol. We did that for increase possibility of exist our part in backbone.
1002 1005 can lig

1002 up>>3 uL
1005 down>>3 uL
can>>1 uL
dH2O>>10 uL

1002 1005 sht lig

K+sht>>1 uL
1002 1005 K1Y>>5uL
dH2O>>11 uL
1002 1005 K1X dig
10 uL plasmid            >>>>>It’s liquid culture can’t pass double antibiotic test but we did it’s double EcoR1,Pst1                             digestion  for see what can be plasmid in this culture 32.5 uL dH2O
Digestion which show below, has been made for confirmation.
K+sht dig
4.2 uL plasmid
EcoR1,Pst1
38.3 uL dH2O

1002 1005 K dig.
7.5 uL plasmid
EcoR1,Pst1
35 uL dH2O

Can dig.
4.7 uL plasmid
EcoR1,Pst1
37.8 uL dH2O

http://2011.igem.org/wiki/images/8/8c/21%2C08%2C11.pdf
  http://2011.igem.org/wiki/images/1/13/21.08.11.png

23.08.11


http://2011.igem.org/File:24.08.11.png
http://2011.igem.org/wiki/images/c/c9/24%2C08%2C11.pdf

24.08.11


These are passed from couple antibiotic experiment which made yesterday:
*K143053+1006+K A1
*K143053+1006+K A2
*K143053+1006+K  A3
*K143053+1006+K  A4
*1001+1005+K  X
*1001+1005+K  Y
*1001+1006+K  B1
*1001+1006+K   B2
*J04500+1006+K   B3
*J04500+1006+K   B4
*1003+1006+K    A1
*1003+1006+K A4
*1004+1006+K   B1
*1004+1005+K  B2
*1002+1006+K   B4
We made plazmid isolation , digestion(ecori1_ pst)and electrophoresis ..

    http://2011.igem.org/wiki/images/2/24/24.08.11_2.pdf

25.08.11


http://2011.igem.org/wiki/images/4/47/25.08.11.png
  http://2011.igem.org/wiki/images/5/58/25%2C08%2C11.pdf

26.08.11


27.08.11


Transformasyondan sonra sıvı kültüre konulan K541_596 (222)in
plasmid isolation u yapıldı jel de yürütüldü fakat iyi görüntü alınamadı.
Nedeni 12ml lik jel hazırlanmasından ya da içinde çok çeşitli plasmid olmasından dolayı olabilir.
K541_925A nın plasmid isolation u yapıldı. Jelde yürütüldü.
http://2011.igem.org/wiki/images/a/a3/27.08.11.png

http://2011.igem.org/wiki/images/6/62/27%2C08%2C2011.pdf
http://2011.igem.org/wiki/images/5/5c/27%2C08%2C2011_2.pdf