Team:Cambridge/Notebook/30 June 2011

From 2011.igem.org

Loading...
OVERVIEW
home


June
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
July
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
August
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Thursday June 30th

A morning exercise of playing around with Arduino boards (see http://en.wikipedia.org/wiki/Arduino for those unfamiliar). The engineers/mathmo's/physicists seem relieved to be on familiar territory for a change. That all changes in the afternoon, when work begins on our mini-project, designed primarily to acquaint the physical types with the vagaries of performing complicated operations on invisible substances. A GFP fusion is to be made to a protein of interest and expressed, to be visualised by fluorescence/confocal microscopy. Quite some time is spent trawling the literature (and the biologists' quite impressive memory of cellular components) to find an interesting target. The mini-project is particularly exciting, since we'll be using the powerful and fairly novel technique of Gibson assembly (well worth looking-up if unfamiliar). More details on the mini-project are available here.

Our main project thoughts are strewed upon post-its upon a large table - still no single idea cool enough to work on, but we're confident that more than one will distill from the chaos next week.