"
Team:British Columbia/Notebook/20 July 2011
From 2011.igem.org
 |
 |
 |
 |
 |
July 20 2011
beta-pinene
- PCR off the synthase using yeast primers (contains XhoI and SpeI restriction enzyme sites) in order to put into the yeast plasmid
- PCR off the synthase using biobrick primers (contains EcoRI, XbaI, SpeI, PstI restriction enzyme sites) in order to put into psb1C3
- Gel verification for both PCRs shows bands at the correct length
(-)-limonene
- PCR off the synthase using yeast primers (contains SpeI and NotI restriction enzyme sites) in order to put into the yeast plasmid
- Gel veriification shows 4 bands - PCR failed
- SDM PCR the synthase using new primers
