File:20110709 MorePromoter combo 2.jpg

From 2011.igem.org

Gel of transformants that should carry esp + rsaA combo insert behind PrsaA run against standard combo. Lane 1: ladder; lanes 2-4: combo from miniprep digest and gel extract insert; lanes 5-7: combo from PCR product insert; lane 8: standard combo without any promoter. None of these transformants show the desired insert. Also, the standard combo gene from gel extraction was more dilute than expected and does not show up very well on this gel.

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current16:18, 12 July 2011Thumbnail for version as of 16:18, 12 July 2011460×509 (17 KB)Aaring (Talk | contribs) (<html> Gel of transformants that should carry <i>esp</i> + <i>rsaA</i> combo insert behind P<sub>rsaA</sub> run against standard combo. Lane 1: ladder; lanes 2-4: combo from miniprep digest and gel extract insert; lanes 5-7: combo from PCR product insert;)

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