06-07-2011
Ideas for modelling
Brainstorm session with Douwe Molenaar and Isoude Kuijper
Steady state model of promoters
- Information needed for model
- Stability
- Transcription
- Translation
- Growth rate
- Information needed for all promoters
- Inducible promoter
- IPTG promoter is also dependable on a lactose receptor/transporter
- Cold shock promoter
- Temperature dependent
- The inducible promoters are also concentration dependent
- Need to look into literature for already available information
The cold chock promoter
- How does this promoter work?
Cryobrick
- What concentration is needed?
- How many targets do the chaperone proteins have?
Enzyme activity
- Enzyme activity is also temperature dependent
Lab Work
- Made a lot of plates and prepared Bba_R0010 and BBa_B0034 for miniprep testing tomorrow