Team:Amsterdam/31 August 2011

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31-08-2011

Transformation check

Only the positive control showed colonies on the plate

  • The construct making will be redone

Constructs

Construct were made according to assembly protocol of Delft 2010.

Digestion

Digestion of vectors with SpeI and PstI:

  • R0010_pSB1A2
  • K206000_pSBA3
  • R0010_B0034_pSB1A2

Digestion of inserts with XbaI and PstI:

  • Shednak
  • CspC
  • Cpn10
  • Cpn60
  • B0034_Cpn10_pSB1A2
  • B0034_Cpn60_pSB1A2
  • B0034_CspC_pSB1A2

Purification

The vectors were purified with size selective purification. The inserts were purified on agarose gel.

Ligation

The following constructs were ligated ON at 16 degrees Celcius:

  • R0010+ B0034 + CspC
  • R0010+ B0034 + Cpn10
  • R0010+ B0034 + Cpn60
  • R0010+ B0034 + Shednak
  • K206000+ B0034 + CspC
  • K206000+ B0034 + Cpn10
  • K206000+ B0034 + Cpn60
  • K206000+ B0034 + Shednak

Growth curves

Still measuring the growth curves for 4 and 8°C